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Protein concentration GSSH (μg / mg prot)=y×Vrv÷Vrv÷Cpr =y÷Cpr Sample weight GSSH (μg /g)= y×Vrv÷(Vrv÷Vsv×W)= y÷W Cellamount GSSH (μg /106 cell)= y×Vrv÷(Vrv÷Vsv×N)= y÷N Solution volume GSSH (μg / mL)= y×Vrv/Vs= 2y N: Cell amount,106； Vrv: Total reaction volume, 0.203 mL； Vsv: The volume of supernatant was added into the reaction system, 20 μL=0.02 mL； W: Sample weight, g